Tag

Part:BBa_K3168008:Design

Designed by: Eva Hanckmann, Harm van der Veer, Claire Michielsen   Group: iGEM19_TU_Eindhoven   (2019-09-17)


HisTag-MBP-TEVsite


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 423
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1246
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 121


Design Notes

Designed for bacterial expression and can be cleaved after protein expression.


Source

Escherichia coli derived maltose-binding protein, which sequence was derived from Addgene plasmid # 60815.

References